HLA class II alleles with susceptibility of leprosy in the Mexican Mestizo population
  
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KeyWord:leprosy, HLA-DR, HLA-DQ, HLA-DRB1*15, HLA-DQB1*05, HLA-DQB1*06
              
AuthorInstitution
Sergio Mercado Ceja Private Practice. Jaime Balmes 11, Torre B, Interior 112-C Plaza Polanco, Los Morales Polanco11510, Miguel Hidalgo, Mexico.
Lucia Rangel Gamboa Department of Ecology of Pathogenic Agents, Research Division,
María Elisa Vega Memije Department of Dermatology
Angélica Olivo Díaz Department of Molecular Biology and Histocompatibility, General Hospital "Dr. Manuel Gea Gonzalez", Mexico City 14080, Mexico
Julio Granados Department of Transplantation, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City 14080, Mexico.
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Abstract:
      Leprosy is a chronic, infectious disease, caused by Mycobacterium leprae, Mycobacterium lepromatosis or both, which affects the peripheral nervous system and the skin. Activation of cellular immunity in infected individuals depends on antigen recognition, which involves relevant HLA-Class II alleles. Therefore, the objective of this study was to determine HLA-Class II allele frequencies (HLA-DRB1and-DQB1)in Mexican Mestizo leprosy patients and compare themwith healthy controls, in order to define their role in the genetic susceptibility to this infection.The genomic DNA of each participant was obtained from peripheral blood, using the salting-out method. PCR amplification and hybridization ofHLA-class II alleleswas made by PCR-SSO. The results showed that frequencies of HLA-DRB1*15(Pc =0.003, OR=3.3 95%CI=1.53-7.33), HLA-DQB1*05(Pc =0.00003, OR=6.03 95%CI=2.49-14.61) and HLA-DQB1*06 (Pc =0.007, OR=2.89, 95%CI=1.38-6.04)were significantly higher among leprosypatients than those of healthy controls. The study suggests that HLA-DRB1*15, HLA-DQB1*05, and HLA-DQB1*06 are associated with leprosy susceptibility in the Mexican Mestizo population.
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