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| Serological and molecular analysis of ABO and Rh blood group chimeras |
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| DOI：10.46701/APJBG.2018042018137 |
| KeyWord:blood group chimera, blood transfusion, ABO, Rh |
| Author | Institution |
| Xiaofei Li |
Beijing Red Cross Blood Center, Beijing 100088, China; |
| YuShiang Lin |
Jiangsu Zhongji Wantai Biopharmaceutical Co., Ltd., Jiangyin 214400, Jiangsu, China; |
| Tingting Liu |
Beijing Red Cross Blood Center, Beijing 100088, China; |
| Ye Zhang |
Beijing Red Cross Blood Center, Beijing 100088, China; |
| Guangyan Zhuang |
Beijing Red Cross Blood Center, Beijing 100088, China; |
| Tianhong Miao |
Beijing Red Cross Blood Center, Beijing 100088, China; |
| Zhiyuan Xu |
Beijing Red Cross Blood Center, Beijing 100088, China; |
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| Abstract: |
| The serological examination, blood transfusion strategies and the molecular analysis to blood group chimera were conducted to demonstrate existent of chimera in blood group. The blood grouping of ABO or/and RhD, newborn red blood cells separated by capillary centrifugation. Aabsorption tests and DTT treated agglutination erythrocyte tests were implemented in four patients. Further molecular biological research was conducted on one patient's sample. The results showed that for patient 1: ABO blood group was AB/B chimera, Rh blood cells contained the RhCE chimera gene; Patient 2: Rh blood cells contained the RhD chimera gene; Patient 3: ABO blood group was AB/B chimera, Rh blood cells contained the RhD chimera gene; Patient 4: ABO blood group was O/B chimera, Rh blood cells contained the RhCE chimera gene. The study suggests that the individuals categorized as chimeras are likely to be more common than existing literature reports. According to the serological tests, in the absence of a history of recent blood transfusion or disease to cause reduced antigen, the phenomena of hybrid aggregation of the ABO and Rh blood system were the main feature. In terms of transfusion strategy, the selection of ABO and Rh blood groups should be depended on the group of cells with more antigens. |
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