Analysis of factors related to ELISA-HBsAg negative and HBV DNA positive blood donors
  
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KeyWord:nucleic acid technology, ELISA, risk factor, blood donor
              
AuthorInstitution
Ziyi He Department of Research Transfusion Laboratory, Dongguang Blood Center, Dongguan 523930, Guangdong,China.
Qing Wang Department of Research Transfusion Laboratory, Dongguang Blood Center, Dongguan 523930, Guangdong,China.
Shaobin Chen Department of Research Transfusion Laboratory, Dongguang Blood Center, Dongguan 523930, Guangdong,China.
LinYu Department of Research Transfusion Laboratory, Dongguang Blood Center, Dongguan 523930, Guangdong,China.
Bitao Huang Department of Research Transfusion Laboratory, Dongguang Blood Center, Dongguan 523930, Guangdong,China.
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Abstract:
      This report analyzed factors relating to ELISA-HBsAg and Hepatitis B virus (HBV) DNA in blood donors. To provide a reference for an accurate screening model for HBV infection in donated blood, we collected rel-evant information from 124 blood donors testing ELISA-HBsAg negative and HBV DNA positive in 2017, including ELISA-HBsAg Max s/co, gender, age, residence, education level, blood donation record and alanine aminotransferase(ALT) value. Meanwhile, 99 blood donors with the results ELISA-HBsAg negative and HBV DNA negative were randomly selected as control. Univariate logistic analysis was conducted for possible correlation factors, then multivariate logistic regression analysis was performed for statistically significant observation indicators. The results showed that the Ct value of HBV DNA mixed test in the observation group donor was higher than that of HBV DNA single test (P<0.05). If one of the two ELISA-HBsAg s/co values was within the range of 0.259 to 0.304, the chance of HBV DNA positive was increased. Univariate logistic regression analysis showed that ELISA-HBsAg Max s/co, gender, age, blood donation history and ALT value were all risk factors in the observation group. Multivariate logistic regression analysis showed that ELISA-HBsAg Max s/co, (OR=5352.448, P<0.05), age (OR= 4.527, P<0.05) and blood donation history (OR=0.441) were risk factors. The study concluded that ELISA-HBsAg Max s/co, age and number of blood donations are risk factors for ELISA-HBsAg negative and HBV DNA positive blood donors, women or donors under 26 years of age had the lowest risk.
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