Application of a multiplex PCR genotyping assay of 31 red blood cell antigens for establishment of a panel of reference DNA in China
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KeyWord:blood group genotyping, multiplex PCR, reference DNA, Chinese population
Yuping Chen Jiangsu LIBO Medicine Biotechnology Co., Ltd., Jiangyin, Jiangsu, 214400,China;
Tongmao Zhao National Institutes of Health, Bethesda, Maryland, 20852, USA.
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      DNA based blood group genotyping has been widely used in clinical blood transfusions, and a number of different molecular blood group testing methods have been developed, including the detection of single nucleotide polymorphism (SNP) and genomic DNA sequencing. As the molecular bases of blood groups can differ widely between ethnic groups, a set of reference DNAs, especially for the Chinese population, is required for the development and validation of these methods, and for their optimal use in routine practice in China. In this study, a total of 100 DNA samples obtained from 60 established cell lines and 40 Chinese blood donors were typed for 31 red blood cell antigens of 13 blood group systems using a multiplex polymerase chain reaction (PCR) assay. Finally, nine DNA samples were selected to establish a panel of reference DNA that included M, N, S, s, Mur, Lua, Lub, Aua, Aub, K, k, Fya, Fyb, Jka, Jkb, Dia, Dib, Sc1, Sc2, Doa, Dob, Coa, Cob, Kna, Knb, Inb, Vel antigens and Fy (a-b-) null phenotype.